DNA metabarcoding

Who's who in the zoo

Creating a dashboard for visualizing zooplankton in the Indian Ocean

Metabarcoding of marine zooplankton in South Africa

Metabarcoding is an emerging method in which DNA barcoding is combined with next-generation sequencing to determine the biodiversity of taxonomically complex samples. We assessed the current state of DNA barcode reference databases for marine …

The design and testing of mini-barcode markers in marine lobsters

Full-length mitochondrial cytochrome c oxidase I (COI) sequence information from lobster phyllosoma larvae can be difficult to obtain when DNA is degraded or fragmented. Primers that amplify smaller fragments are also more useful in metabarcoding …

DNA metabarcoding zooplankton in marine protected areas on the east coast of South Africa

Investigating DNA metabarcoding as a powerful new tool to assess the biodiversity of zooplankton, particularly in Marine Protected Areas. DNA metabarcoding is a molecular method that uses universal genetic markers to obtain information on the various sequences of DNA from all organisms in a sample, such as in the mix of plankton obtained from towing fine-mesh nets. The DNA is matched to reference (known) sequences stored on online databases, such as GenBank and the Barcode of Life.